DNA Melting Temperature (Tm) Calculator

Estimate melting temperature (Tm) and GC content of a short DNA sequence (educational approximation).

DNA sequence (A, T, G, C)

Enter a short sequence (no spaces or only A/T/G/C).

Calculation method

Wallace is common for short primers.

Salt correction

Adds simple salt adjustment.

Precision

Display rounding.

Length

—

GC content

—

Melting temperature (Tm)

—

Simplified model for educational purposes. Real lab conditions require precise thermodynamic calculations.